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Unconjugated Primary Antibodies



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Catalog #1: bsm-51673m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51556m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ERK2 monoclonal antibody, unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK2 antibody (bsm-51555M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-51555m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: NIH\/3T3 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51505m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51756m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51759m

Applications: WB

Reactivity: Human, Mouse, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-51758m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51757m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Catalog #1: bsm-51753m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51752m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51751m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: CCRF-CEM cell lysates; Lane 4: CHO cell lysates probed with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-51750M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: DU145 cell lysates; Lane 2: U-251MG cell lysates probed with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-51750M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51750m

Applications: WB

Reactivity: Human, Hamster

Conjugate: Unconjugated

Lane 1: HL-60 cell lysates; Lane 2: THP-1 cell lysates; Lane 3: K562 cell lysates probed with CARD9 Monoclonal Antibody, Unconjugated (bsm-51749M) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K62 cell lysates; Lane 2: HL-60 cell lysates probed with CARD9 Monoclonal Antibody, Unconjugated (bsm-51749M) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51749m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51748m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates; Lane 2: Human Lung lysates; Lane 3: Human Liver lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Kidney lysates; Lane 6: Rat Kidney lysates probed with SULT1A1 Monoclonal Antibody, Unconjugated (bsm-51747M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates probed with SULT1A1 Monoclonal Antibody, Unconjugated (bsm-51747M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51747m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51746m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates; Lane 2: MDA-MB-468 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mcf-7 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51745m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51743m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51742m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human LOVO cell lysates probed with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-51741M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with PTGS2\/Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-51741M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51741m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51740m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51738m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51737m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51733m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51716m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51726m

Applications: WB

Reactivity: Human, Recombinant protein

Conjugate: Unconjugated

Catalog #1: bsm-51724m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51723m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51721m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 cell lysates probed with RAB5B Monoclonal Antibody, Unconjugated (bsm-51720M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: NIH\/3T3 cell lysates probed with RAB5B Monoclonal Antibody, Unconjugated (bsm-51720M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51720m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51715m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51714m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Raji cell lysates probed with HLA-F Monoclonal Antibody, Unconjugated (bsm-51712M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: HL-60 cell lysates probed with HLA-F Monoclonal Antibody, Unconjugated (bsm-51712M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51712m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51706m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51705m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51702m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51699m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51698m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51697m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat cell lysates; Lane 2: HepG2 cell lysates probed with RBX1 Monoclonal Antibody, Unconjugated (bsm-51695M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates probed with RBX1 Monoclonal Antibody, Unconjugated (bsm-51695M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51695m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51694m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51692m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51691m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51690m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51689m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51685m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51688m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51687m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated