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Unconjugated Primary Antibodies



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Catalog #1: bsm-30015m-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-48086r-100ul

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-42585r-100ul

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-42586r-100ul

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-42584r-100ul

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-17107r-100ul

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney tissue labeled with Anti CD30\/TNFRSF8\/CD153 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: A431 cell lysates probed with CD30 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2495r-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Unconjugated

Flow cytometric analysis of SH-SY5Y cells with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54144r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF\/CRH Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF\/CRH Polyclonal Antibody (bs-0382R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CRF Polyclonal Antibody (bs-0382R) at 1:400 overnight at 4\u00b0C, followed by a FITC conjugated secondary and DAPI staining.

Catalog #1: bs-0382r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-90295r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-90294r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-TSHR Polyclonal Antibody (bs-0460R)at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse thymocytes probed with Rabbit Anti-TSHR Polyclonal Antibody, Unconjugated (bs-0460R) (green) at 1:100 for 60 minutes followed by a FITC conjugated secondary compared to control cells (blue).

Catalog #1: bs-0460r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70705m

Applications: WB, IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60861r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60841m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human spleen ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Nestin monoclonal antibody, unconjugated (bsm-60784M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60784m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD99 Monoclonal Antibody, Unconjugated (bsm-60792R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD99 Monoclonal Antibody, Unconjugated (bsm-60792R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60792r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60787r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60785r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD16a Monoclonal Antibody, Unconjugated(bsm-60782R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD16a Monoclonal Antibody, Unconjugated(bsm-60782R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD16a Monoclonal Antibody, Unconjugated(bsm-60782R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD16a Monoclonal Antibody, Unconjugated(bsm-60782R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60782r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60742m

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-55535m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-54156r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with FGF21 monoclonal antibody, unconjugated (bsm-52978R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-52978r

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human placenta cells lysates probed with CD41 (7C8) Monoclonal Antibody (bsm-52912R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD41\/ITGA2B Monoclonal Antibody, Unconjugated (bsm-52912R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52912r

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation TARDBP Monoclonal Antibody, Unconjugated (bsm-52949R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation TARDBP Monoclonal Antibody, Unconjugated (bsm-52949R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation TARDBP Monoclonal Antibody, Unconjugated (bsm-52949R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation TARDBP Monoclonal Antibody, Unconjugated (bsm-52949R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52949r

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Tau monoclonal antibody, unconjugated (bsm-52310R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau Monoclonal Antibody, Unconjugated(bsm-52310R) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau Monoclonal Antibody, Unconjugated(bsm-52310R) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau Monoclonal Antibody, Unconjugated(bsm-52310R) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-52310r

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

\tParaformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad5 Monoclonal Antibody, Unconjugated(bsm-52226R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad5 Monoclonal Antibody, Unconjugated(bsm-52226R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad5 Monoclonal Antibody, Unconjugated(bsm-52226R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad5 Monoclonal Antibody, Unconjugated(bsm-52226R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52226r

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela (H) cells were treated with or without UV (40 mJ\/cm2) for 30 min and anisomycin (25 \u03bcg\/mL) for 30 min , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-HSP27 (Ser82) monoclonal antibody, unconjugated (bsm-52168R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela( treated with 200J\/m2 UV-C then recovery for 30 min) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-p-Hsp27 (S82) antibody (bsm-52168R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-52168r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with HDAC2 monoclonal antibody, unconjugated (bsm-52083R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC2) monoclonal Antibody, unconjugated (bsm-52083R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-HDAC2 antibody (bsm-52083R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-52083r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52076r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52005r

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-4713m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-43685m

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43179m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human IGFBP3 protein, His (HEK293)(bs-43124P) probed with IGFBP3 Monoclonal Antibody, Unconjugated (bsm-43124M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IGFBP3) Monoclonal Antibody, Unconjugated (bsm-43124M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IGFBP3) Monoclonal Antibody, Unconjugated (bsm-43124M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IGFBP3) Monoclonal Antibody, Unconjugated (bsm-43124M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-43124m

Applications: IHC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-43105m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (bsm-42319M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (bsm-42319M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Human Lambda Light Chain monoclonal antibody, unconjugated (bsm-42319M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-42319m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human HER2 protein, N-His(bs-42263P) probed with ERBB2\/HER2 Monoclonal Antibody, Unconjugated (bsm-42263M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HER2) Monoclonal Antibody, Unconjugated (bsm-42263M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HER2) Monoclonal Antibody, Unconjugated (bsm-42263M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HER2) Monoclonal Antibody, Unconjugated (bsm-42263M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-42263m

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human FOXP3 protein, N-His(bs-42173P) probed with FOXP3 Monoclonal Antibody, Unconjugated (bsm-42173M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-FOXP3 antibody (bsm-42173M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-42173m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-41804m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-41802m

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41730m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(bsm-41722M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(bsm-41722M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(bsm-41722M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(bsm-41722M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-41722m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41463m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (bsm-41335R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (bsm-41335R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (bsm-41335R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (bsm-41335R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-41335r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41304m

Applications: WB, IHC, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human Pepsinogen C \/ PGC protein, His(bs-41229P) probed with PGC Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-41229m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated