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Unconjugated Primary Antibodies



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Catalog #1: bs-60022R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-60043R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Tissue:Human cervical carcinoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60277R
Tissue:mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60277R
Tissue:rat colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60277R
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (JCHAIN) Monoclonal Antibody, Unconjugated (bsm-60277R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60277r

Applications: IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60236R
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60236R

Catalog #1: bsm-60236r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HBA1 Monoclonal Antibody, Unconjugated (bsm-60287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HBA1 Monoclonal Antibody, Unconjugated (bsm-60287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HBA1 Monoclonal Antibody, Unconjugated (bsm-60287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HBA1 Monoclonal Antibody, Unconjugated (bsm-60287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60287r

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed HL-60 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ICOS) monoclonal Antibody, unconjugated (bsm-60227R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HL-60(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-ICOS antibody (bsm-60227R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with ICOS monoclonal antibody, unconjugated (bsm-60227R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60227r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60297R
Tissue: mouse colon \r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60297R
Tissue: Rat stomach\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60297R

Catalog #1: bsm-60297r

Applications: IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Tissue:Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60020R
25 ug total protein per lane of various lysates (see on figure) probed with ERBB2\/HER2 monoclonal antibody, unconjugated (bsm-60020R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60020r-100ul

Applications: WB

Reactivity:

Conjugate: Unconjugated

Tissue:human pancreas\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:4000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60010R
Tissue:mouse pancreas\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:4000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60010R
Tissue:rat pancreas\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:4000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60010R
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated(bsm-60010R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-60010r

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 66 kDa\r\nObserved MW: 66 kDa\r\n
Cell line: HepG2\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60006R
25 ug total protein per lane of various lysates (see on figure) probed with Glypican 3 monoclonal antibody, unconjugated (bsm-60006R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60006r

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-60003R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-60003R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-60003R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-60003R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60003r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (bsm-60002R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (bsm-60002R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (bsm-60002R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (bsm-60002R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60002r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with muscle Actin polyclonal antibody, unconjugated (bs-13947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-13947r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-23312R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

NIH3T3 cells were stained with the Ash2 antibody (bs-53055R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two is shown on the right.
To determine the titer, an ELISA was performed using a serial dilution of the Ash2 antibody (bs-53055R). The wells were coated with the peptides used for immunization of the host rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:24,000.

Catalog #1: bs-53055R

Applications: WB, ELISA, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-53087R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53070R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53142R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53141R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51079R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51067R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51055R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51058R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51068R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51087R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51088R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51070R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51146R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51080R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51141R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51118R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51093R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51104R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51084R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51060R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51128R

Applications: WB, ELISA, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51086R

Applications: ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51047R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51052R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51077R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51073R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51140R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51094R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51089R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51090R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51081R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51145R

Applications: ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51144R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated