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Western Blot Antibodies



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Catalog #1: bs-10557R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-20372R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-20281R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-10551R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-20281R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-10551R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-20273R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-10551R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20273R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-10540R-Biotin

Applications: WB, ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bs-20351R-HRP

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-20272R-HRP

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-20351R-Biotin

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-20272R-Biotin

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-10442R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-10442R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-20269R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-20286R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-20286R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1028R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-20269R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-1028R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-20285R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-20190R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-20285R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-10248R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-20190R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Hela lysates probed with TRIM23 Polyclonal Antibody, Unconjugated (bs-20285R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TRIM23 Polyclonal Antibody, Unconjugated (bs-20285R) at 1:500 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20285R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-10248R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-20190R

Applications: WB, ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20177R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-20181R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-20177R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-20181R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-20594R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Cow

Conjugate: HRP

Catalog #1: bs-20594R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Biotin

Catalog #1: bs-1316R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1316R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10610R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-10610R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-3687R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3687R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0774R-Biotin

Applications: WB

Reactivity: Mouse

Conjugate: Biotin

Western blot was performed on whole cell (25\u03bcg, lane 1) and histone extracts (15\u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K5ac Polyclonal Antibody (bs-53099R). Antibody was diluted 1:500 in TBS-Tween containing 5% skim milk.
Dot Blot analysis was performed with peptides containing a variety of histone modifications and the unmodified H4. 0.2-100 pmol of the respective peptides were spotted on a membrane. H4K5ac Polyclonal Antibody (bs-53099R) was used at 1:5,000 dilution. Result shows high specificity of the antibody for the modification of interest.
ChIP were performed using HeLa cells using H4K5ac Polyclonal Antibody (bs-53099R). Chromatin was sheared from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10\u03bcg per ChIP experiment was analyzed. IgG (2\u03bcg\/IP) was used as negative IP control. Quantitative PCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH as positive controls, and for the inactive MYOD1 gene and Sat2 satellite repeat region used as negative controls. Result shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ELISA was performed using a serial dilution of the\r\nH4K5ac Polyclonal Antibody (bs-53099R) against peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,900.

Catalog #1: bs-53099R

Applications: WB, ELISA, IHC-P, IF(ICC), dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Histone extracts of HeLa cells (15 \u03bcg) were analysed by Western blot using the H4K20me1 Polyclonal Antibody (bs-53035R) diluted 1:750 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
To check the specificity of H4K20me1 Polyclonal Antibody (bs-53035R) at 1:20,000 dilution, a Dot Blot was performed with peptides containing different modifications of histone H4 and the unmodified sequence. 0.2 to 100 pmol concentration of peptides were spotted on to a membrane. Experiment shows specificity of the antibody for the modification of interest.
To determine the titer, an ELISA was performed using a serial dilution of the H4K20me1 Polyclonal Antibody (bs-53035R), to detect peptide containing histone modification of interest. By plotting the absorbance against antibody dilutions, titer of the antibody was estimated to be 1:8,000.
ChIP were performed using U2OS cells using H4K20me1 Polyclonal Antibody (bs-53035R). Chromatin was sheared from 1.6 million cells. A titration of the antibody consisting of 2, 5, 10 and 15\u03bcl per ChIP experiment was analyzed. IgG (5\u03bcg\/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for the coding region of the myogenic differentiation gene (MYOD) which is inactive at normal conditions. This figure shows the recovery, expressed as a % of input.

Catalog #1: bs-53035R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Others

Conjugate: Unconjugated

Nuclear extracts of HeLa cells (40 \u03bcg) were analysed by Western blot using p53 Polyclonal Antibody (bs-53127R) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. Followed by secondary antibody incubation.
ChIP assays were performed using human U2OS cells, treated with camptothecin, p53 Polyclonal Antibody (bs-53127R) and optimized PCR primer sets for QPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 \u03bcg of the p53 Polyclonal Antibody (bs-53127R). The IP\u2019d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer\u2019s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The peak distribution along the X-chromosome and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of p53 Polyclonal Antibody (bs-53127R), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:308,000. Crude serum (blue), purified antibody (pink), flow through (yellow).

Catalog #1: bs-53127R

Applications: WB, ELISA, IF(ICC), ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23118R-HRP

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-23118R-Biotin

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Biotin