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Western Blot Antibodies



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Catalog #1: bsm-61880r-rbitc-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (eIF4A3) monoclonal Antibody, unconjugated (bsm-61883R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-eIF4A3 antibody (bsm-61883R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (bsm-61883R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (bsm-61883R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61883r

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61882r-fitc-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-61880r-bf647-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61882r-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-61880r-percp-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61882r-bf594-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61883r-bf488-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61882r-percp-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61880r-bf405-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61882r-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-61880r-apc-cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bsm-61883r-bf350-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61880r-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-61882r-bf647-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61882r-apc-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-61878r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-61880r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-61880r-bf555-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61882r-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-61880r-bf488-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61878r-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-61878r-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61878r-fitc-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-61878r-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-61878r-pe-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-61878r-bf594-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61878r-bf350-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61878r-bf750-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61878r-bf555-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61878r-cy5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-61878r-bf647-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61878r-bf488-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61878r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-61878r-bf405-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61878r-cy3-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-61877r-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: RBITC

Catalog #1: bsm-61878r-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-61878r-apc-cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bsm-61878r-apc-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: APC

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (eIF4A1) monoclonal Antibody, unconjugated (bsm-61878R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with eIF4A1 monoclonal antibody, unconjugated (bsm-61878R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-61878r

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-61877r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-61877r-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61877r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-61877r-percp-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bsm-61877r-pe-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bsm-61877r-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-61877r-bf488-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-488