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Formalin-fixed and paraffin embedded: rat spinal cord tissue labeled with Anti-Neurofascin Polyclonal Antibody, Unconjugated (bs-0289R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neurofascin Polyclonal Antibody, Unconjugated (bs-0289R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse cerebellum lysates probed with Neurofascin Polyclonal Antibody, unconjugated (bs-0289R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Rat Cerebellum lysates probed with Neurofascin Polyclonal Antibody, Unconjugated (bs-0289R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0289R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BACE1\/ASP2 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates probed with Anti BACE1\/ASP2 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 51kD. Observed band size:51kD.\\n
Mouse muscle lysates probed with Rabbit Anti-BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0164R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0360G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DDC\/DOPA Decarboxylase Polyclonal Antibody, Unconjugated (bs-0180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse adrenal gland tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat adrenal gland tissue lysates ; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human A549 cell lysates probed with DOPA Decarboxylase Polyclonal Antibody,Unconjugated (bs-0180R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0180R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EAAT2 ) Polyclonal Antibody, Unconjugated (bs-49183R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EAAT2 ) Polyclonal Antibody, Unconjugated (bs-49183R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EAAT2 ) Polyclonal Antibody, Unconjugated (bs-49183R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EAAT2 ) Polyclonal Antibody, Unconjugated (bs-49183R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-49183r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation withIL-4 Monoclonal Antibody, Unconjugated(bsm-54002R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation withIL-4 Monoclonal Antibody, Unconjugated(bsm-54002R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation withIL-4 Monoclonal Antibody, Unconjugated(bsm-54002R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with IL-4 monoclonal antibody, unconjugated (bsm-54002R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54002r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0426R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with MBP polyclonal antibody, HRP conjugated (bs-24827R-HRP) at 1:1000 dilution and 4\u00b0C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody (bs-24827R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody (bs-24827R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody (bs-24827R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bs-24827R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P53(Mono Methyl Lys370) Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P53(Mono Methyl Lys370) Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P53(Mono Methyl Lys370) Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Urinary bladder lysates; Lane 2: Mouse Skin lysates probed with P53 Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33209M

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human U251 lysates probed with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Catalog #1: bs-20445R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human SP2\/0 lysates; Lane 2: Human A673 lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-20751R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Skeletal muscle lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-20751R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20751R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti- WT-1\/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD
Formalin-fixed and human kidney tissue labeled with Anti-WT-1\/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6983R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11961r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 mouse embryo lysates L2 mouse liver lysates probed with Anti CGP-39\/YKL-40 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (bs-1093R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Lung tissue lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1093R

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K79) Monoclonal Antibody, Unconjugated (bsm-33097M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K79) Monoclonal Antibody, Unconjugated (bsm-33097M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K79) Monoclonal Antibody, Unconjugated (bsm-33097M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K79) Monoclonal Antibody, Unconjugated (bsm-33097M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33097M

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rat Lung lysates;Lane 2: Rat Spleen lysates probed with Apelin Receptor Polyclonal Antibody, Unconjugated (bs-21310R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Thymus lysates probed with Apelin Receptor Polyclonal Antibody, Unconjugated (bs-21310R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Apelin Receptor Polyclonal Antibody, Unconjugated (bs-21310R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human K562 cell lysates probed with Apelin Receptor Polyclonal Antibody, Unconjugated (bs-21310R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-21310R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-1004R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

SH-SY-5Y cells lysates; Probed with Gli1 (5C3) Monoclonal Antibody (bsm-52864R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse fallopian tube tissue incubated with Gli1 (5C3) Monoclonal Antibody (bsm-52864R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat testis tissue incubated with Gli1 (5C3) Monoclonal Antibody (bsm-52864R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52864r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52837r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52647r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD27 monoclonal antibody, unconjugated (bsm-54318R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54318r-TR

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with CD4 (65H6) Monoclonal Antibody (bsm-52469R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Jurkat cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD4 Monoclonal Antibody(bsm-52469R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52469r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EpCAM (6H5)) Monoclonal Antibody, Unconjugated (bsm-52417R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EpCAM (6H5)) Monoclonal Antibody, Unconjugated (bsm-52417R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EpCAM (6H5)) Monoclonal Antibody, Unconjugated (bsm-52417R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-52417R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-52417r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Lane 2: PC-3M Cells; Lane 3: A549 Cells; Probed with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HepG2 cells with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Catalog #1: bsm-54417r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with COX2\/Cyclooxygenase 2 (14D5) Monoclonal Antibody (bsm-52502R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52502r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54170r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

araformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GBA Monoclonal Antibody, Unconjugated(bsm-52907R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
araformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GBA Monoclonal Antibody, Unconjugated(bsm-52907R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
araformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GBA Monoclonal Antibody, Unconjugated(bsm-52907R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
araformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GBA Monoclonal Antibody, Unconjugated(bsm-52907R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52907r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U-2OS cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Hela cell lysates probed with Id1 (5F1) Monoclonal Antibody, Unconjugated (bsm-54101R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ID1) monoclonal Antibody, Unconjugated (bsm-54101R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ID1 Monoclonal Antibody(bsm-54101R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ID1 Monoclonal Antibody, Unconjugated (bsm-54101R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54101r

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Brain Lysates; Lane 2: Human K562 cell Lysates. Probed with Acetyl CoA Carboxylase 1(ACC1) monoclonal Antibody, unconjugated (bsm-52486R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACACA Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACACA Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACACA Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52486r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-52392r-TR

Applications: WB, IHC-P, IHC-F, IF(ICC), IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDX2 Monoclonal Antibody, Unconjugated(bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52348r

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52264r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ERK1\/2 (3A12) Monoclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Tissue\/cell:A549 cell;4% Paraformaldehyde-fixed;Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ERK1\/2) monoclonal Antibody, Unconjugated (bsm-52259R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated(bsm-52259R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated(bsm-52259R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52259r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ISX\/Intestine specific homeobox Polyclonal Antibody, Unconjugated (bs-18172R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse small intestine lysates; Lane 2: Mouse large intestine lysates probed with ISX\/Intestine specific homeobox Polyclonal Antibody, Unconjugated (bs-18172R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Lovo cell lysates; Lane 2: Human SW480 cell lysates probed with ISX\/Intestine specific homeobox Polyclonal Antibody, Unconjugated (bs-18172R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-18172R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

TT lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A431 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U251 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Stomach tissue probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-17422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

ICC staining of c-Jun(S63) in MCF-7 cells (green) using c-Jun(S63) (6C2) Monoclonal Antibody (bsm-52141R). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
ICC staining using c-Jun(S63) (6C2) Monoclonal Antibody followed by A549 conjugated secondary antibody staining (green), and nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nc-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nc-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52141r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11519R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Mouse cerebellum lysates probed with JNK1 + JNK2 + JNK3 (T183 + T183 + T221) Polyclonal Antibody, Unconjugated (bs-4163R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1 + JNK2 + JNK3 (T183 + T183 + T221) Polyclonal Antibody, Unconjugated (bs-4163R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1 + JNK2 + JNK3 (T183 + T183 + T221) Polyclonal Antibody, Unconjugated (bs-4163R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Mouse Large intestine lysates; Lane 5: Mouse Uterus lysates; Lane 6: Mouse NIH\/3T3 cell lysates; Lane 7: Human A431 cell lysates probed with JNK1 + JNK2 + JNK3 Polyclonal Antibody, Unconjugated (bs-4163R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4163R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33188M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (ascites of bsm-33188M 3F9) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
The NIH\/3T3 (M) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-alpha smooth muscle Actin antibody (bsm-33188M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-40296G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33188M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33188m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IRF5) monoclonal Antibody, unconjugated (bsm-52117R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-IRF5 antibody (bsm-52117R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with IRF5 monoclonal antibody, unconjugated (bsm-52117R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IRF5 Monoclonal Antibody, Unconjugated (bsm-52117R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52117r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 4(2F9)) Monoclonal Antibody, Unconjugated (bsm-52062R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 4(2F9)) Monoclonal Antibody, Unconjugated (bsm-52062R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 4(2F9)) Monoclonal Antibody, Unconjugated (bsm-52062R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 4(2F9)) Monoclonal Antibody, Unconjugated (bsm-52062R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-52062r-TR

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 10 (1D8) Monoclonal Antibody, Unconjugated (bsm-52052R) at 1:1000 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 10 (1D8) Monoclonal Antibody, Unconjugated (bsm-52052R) at 1:1000 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10\/Cytokeratin 10 Monoclonal Antibody, Unconjugated(bsm-52052R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10\/Cytokeratin 10 Monoclonal Antibody, Unconjugated(bsm-52052R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52052r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rela-KO cell lysates; Lane 2: Hela cell lysates probed with NFKB p65 (7G6) Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with NFKB p65 monoclonal antibody, unconjugated (bsm-33117M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, unconjugated (bsm-33117M) 1:50, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-40296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated(bsm-33117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33117m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Antigen: bs-6125P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
L1 rat brain lysates L2 mouse embryo lysates probed with Anti phospho-NBS1(Ser343) Polyclonal Antibody, Unconjugated (bs-6125R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 85kD.Observed band 85kD.

Catalog #1: bs-6125R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Tissue\/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal mouse serum, C-0082) at 37\u00b0C for 20 min; Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (bsm-33042M-AF350) 1:100, 2 hours at 37\u00b0C; PI (red, S0009) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33042m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20831R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: L6; Lane 2: NIH\/3T3; Lane 3: T47D; Lane 4: A2058; Lane 5: HepG2; Lane 6: K562; Lane 7: HeLa Cell lysates; probed with Hsp90 (3C9) Monoclonal Antibody, unconjugated (bsm-51215M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Placenta tissue lysates ; Lane 6: Human U251 cell lysates ; Lane 7: Human Molt-4 cell lysates ; Lane 8: Human HL60 cell lysates ; Lane 9: Human A549 cell lysates probed with HSP90 Monoclonal Antibody, Unconjugated (bsm-51215M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51215m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8388R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated