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Western Blot Antibodies



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Catalog #1: bs-10423r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-10423r-apc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-10423r-apc-cy55-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-12757r-percp-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-12757r-percp-cy55-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-12757r-rbitc-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-12757r-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-12757r-pe-cy55-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-12757r-percp-cy7-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bs-12757r-cy7-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-12757r-hrp-100ul

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-12757r-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-12757r-pe-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bs-12757r-fitc-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-12757r-cy55-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-12757r-cy5-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-12757r-bf647-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-12757r-bf750-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-12757r-cy3-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-12757r-biotin-100ul

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-12757r-bf680-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-12757r-bf594-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-12757r-bf488-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-12757r-bf555-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-12757r-bf405-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-12757r-apc-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: APC

Catalog #1: bs-12757r-bf350-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-12757r-apc-cy7-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: APC-Cy7

HT1080 cell lysates probed with phospho-Vimentin (Ser55) Polyclonal Antibody, Unconjugated (bs-12757R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Vimentin (Ser55) Polyclonal Antibody(bs-12757R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U-87MG cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-12757R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12757r-100ul

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti-P53 protein(wt-p53) Polyclonal Antibody (bs-0033R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
1 rat brain, L2 human colon carcinoma lysates probed (bs-0033R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 53kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of p53 protein in mouse squamous cell cancer (KLN-205 cell line) with BS-0033R antibody (1:50) and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P53 protein(wt-p53) Polyclonal Antibody(bs-0033R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0033r-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12757r-apc-cy55-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-44016r-percp-100ul

Applications: WB

Reactivity: RV

Conjugate: PerCP

Catalog #1: bs-44016r-rbitc-100ul

Applications: WB

Reactivity: RV

Conjugate: RBITC

Catalog #1: bs-44015r-pe-cy7-100ul

Applications: WB

Reactivity: FeHV-1

Conjugate: PE-Cy7

Catalog #1: bs-44016r-pe-cy7-100ul

Applications: WB

Reactivity: RV

Conjugate: PE-Cy7

Catalog #1: bs-44016r-percp-cy55-100ul

Applications: WB

Reactivity: RV

Conjugate: PerCP-Cy5.5

Catalog #1: bs-44016r-cy7-100ul

Applications: WB

Reactivity: RV

Conjugate: Cy7

Catalog #1: bs-44016r-percp-cy7-100ul

Applications: WB

Reactivity: RV

Conjugate: PerCP-Cy7

Catalog #1: bs-44016r-pe-cy5-100ul

Applications: WB

Reactivity: RV

Conjugate: PE-Cy5

Catalog #1: bs-44015r-bf555-100ul

Applications: WB

Reactivity: FeHV-1

Conjugate: AbBy Fluor-555

Catalog #1: bs-44016r-pe-100ul

Applications: WB

Reactivity: RV

Conjugate: PE

Catalog #1: bs-44016r-fitc-100ul

Applications: WB

Reactivity: RV

Conjugate: FITC

Catalog #1: bs-44016r-bf405-100ul

Applications: WB

Reactivity: RV

Conjugate: AbBy Fluor-405

Catalog #1: bs-44016r-bf680-100ul

Applications: WB

Reactivity: RV

Conjugate: AbBy Fluor-680

Catalog #1: bs-44016r-bf555-100ul

Applications: WB

Reactivity: RV

Conjugate: AbBy Fluor-555

Catalog #1: bs-44016r-bf488-100ul

Applications: WB

Reactivity: RV

Conjugate: AbBy Fluor-488

Catalog #1: bs-44016r-cy3-100ul

Applications: WB

Reactivity: RV

Conjugate: Cy3

Catalog #1: bs-44016r-pe-cy55-100ul

Applications: WB

Reactivity: RV

Conjugate: PE-Cy5.5