Receive 30% off all Monoclonal IHC antibodies with code "MONO-IHC-30"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Western Blot Antibodies



×

Collection Menu
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti CD56\/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CD56\/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse cerebral cortex lysates probed with CD56 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Jurkat cells probed with CD56 Polyclonal Antibody, unconjugated (bs-0805R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

Catalog #1: bs-0805R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0646r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CYP5A1\/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 Lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse liver lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0191R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with anti-Calponin 1\/COLP Polyclonal Antibody (bs-0095R), Unconjugated followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Calponin 1\/COLP Polyclonal Antibody, Unconjugated (bs-0095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Calponin 1\/COLP Polyclonal Antibody, Unconjugated (bs-0095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
H9C2 cells probed with Rabbit Anti-Calponin 1 Polyclonal Antibody (bs-0095R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)

Catalog #1: bs-0095R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-STAT3 (Ser727)) monoclonal Antibody, Unconjugated (bsm-52210R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated(bsm-52210R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated(bsm-52210R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated(bsm-52210R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52210r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RGC32 Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was kindly submitted by Horea Rus of the University of Maryland.\\nParaffin embedded Human kidney tissue; Stained with Rabbit Anti-RGC-32 Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 dilution followed by the RTU Vectastain kit from Vector.
This image was kindly submitted by Horea Rus of the University of Maryland.\\nParaffin embedded Human kidney tissue; Stained with Rabbit Anti-RGC-32 Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 dilution followed by the RTU Vectastain kit from Vector.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-9079R

Applications: IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: H9C2 cell lysates probed with Eph receptor A3 Polyclonal Antibody, Unconjugated (bs-7032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Eph receptor A3 Polyclonal Antibody, Unconjugated (bs-7032R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7032R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1:rat brain lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse Testis lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (bs-6695R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates probed with Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6695R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-SEZ6L Polyclonal Antibody, Unconjugated (bs-6103R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with SEZ6L Polyclonal Antibody, Unconjugated (bs-6103R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6103R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti PRODH Polyclonal Antibody,Unconjugated (bs-5813R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti PRODH Polyclonal Antibody,Unconjugated (bs-5813R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PRODH Polyclonal Antibody, Unconjugated (bs-5813R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5813R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GDF9 Polyclonal Antibody, Unconjugated (bs-4720R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Ovary cell lysates probed with GDF9 Polyclonal Antibody, Unconjugated (bs-4720R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GDF9 Polyclonal Antibody(bs-4720R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4720R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated (bs-4055R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Rat Liver; 40ug loaded into the lane; Probed with PPAR alpha (Ser12) Polyclonal Antibody, Unconjugated (bs-4055R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Heart; Lane 2: Mouse Liver; 40ug loaded into the lane; Probed with PAR alpha (Ser12) Polyclonal Antibody, Unconjugated (bs-4055R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
An ELISA was performed using a serial dilution of PPAR Alpha (Ser12) Polyclonal Antibody (bs-4055R) in antigen-coated wells. The antigen used was a peptide containing the phosphosite of interest. The plot shows the absorbance against the antibody dilution for phosphopeptide antigen and non-phosphorylated peptide antigen.

Catalog #1: bs-4055R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti LATS2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1\/2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1\/2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1\/2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4081R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-UCHL1\/PGP9.5 Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3806R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Catalog #1: bs-2888R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat uterus labeled with Anti MAFbx\/Fbx32 Polyclonal Antibody, Unconjugated (bs2591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Fbx32 Polyclonal Antibody(bs-2591R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2591R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

L1 rat kidney lysates and L2 rat brain lysates lysates probed with Anti- IL-17RB Polyclonal Antibody, Unconjugated (bs-2610R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band: 33kD\/55kD Observed band size: 35kD
Image kindly submitted by Dr. Kang Chen from Wayne University via the Bioss Discovery Program.\\n\\nPositive staining with Human Peripheral Mononuclear Cells using Rabbit Anti-IL-17RB Polyclonal Antibody, ALEXA FLUOR 488 Conjugated (bs-2610R-A488)
Lane 1: Mouse Liver lysates probed with IL-17RB Polyclonal Antibody, Unconjugated (bs-2610R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Uterus tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human MCF-7 cell lysates probed with IL17RB Polyclonal Antibody, Unconjugated (bs-2610R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2610R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti ERK1\/2(p42\/p42 MAPK) Polyclonal Antibody, Unconjugated (bs-2637R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.
A549 cell lysates probed with Rabbit Anti-ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Liver lysates probed with ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell Lysate;Lane 2: Mouse Cerebrum Lysate;Lane 3: Rat Cerebrum Lysate;Lane 4: Mouse Lymph node Lysate;Lane 5: Rat Lymph node Lysate;Lane 6: SiHa cell Lysate;Lane 7: HUVEC cell Lysate probed with ERK1 + ERK2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C\r\n\r\n

Catalog #1: bs-2637R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2501R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1666R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13254R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: U2os lysates; Lane 2: MDA-MB-231 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: 293T cell lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

Catalog #1: bs-11929R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric parietal cells labeled with Anti-Gastrin Polyclonal Antibody, Unconjugated (bs-1189R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Gastrin Polyclonal Antibody, Unconjugated (bs-1189R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat stomach ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Gastrin) Polyclonal Antibody, Unconjugated (bs-1189R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Catalog #1: bs-1189R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-STAT3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody (bs-1141R), (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-STAT3 Polyclonal Antibody (bs-1141R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
RSC96 cells probed with Rabbit Anti-STAT3 Polyclonal Antibody (bs-1141R),6ug, for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC)at 0.5ug(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 Polyclonal Antibody, Unconjugated (bs-1141R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1141R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti PAX3 Polyclonal Antibody, Unconjugated (bs-1097R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 53kD. Observed band size: 53kD
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Rat Muscle tissue lysates probed with PAX3 Polyclonal Antibody, Unconjugated (bs-1097R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1097R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ET-1 Polyclonal Antibody (bs-0188R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat stomach labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat heart muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Big endothelin-1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0188r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SPR Polyclonal Antibody, Unconjugated (bs-0064R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti Substance P Receptor\/NK1R Polyclonal Antibody, Unconjugated (bs-0064R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
HeLa lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, unconjugated (bs-0064R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
U251 lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, unconjugated (bs-0064R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0064r-TR

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with NF-kB p65 (3G1) Monoclonal Antibody (bsm-52305R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with NF-kB p65 (3G1) Monoclonal Antibody (bsm-52305R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (bsm-52305R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (bsm-52305R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.

Catalog #1: bsm-52305r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1867R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-18379R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti CKAP4 Polyclonal Antibody,Unconjugated (bs-6520R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CKAP4 Polyclonal Antibody, Unconjugated (bs-6520R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6520R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti PRSS8 Polyclonal Antibody (bs-6064R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human prostate cancer labeled with Anti-PRSS8 Polyclonal Antibody, Unconjugated (bs-6064R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Muscle lysates; Lane 2: Rat Muscle lysates probed with PRSS8 Polyclonal Antibody, Unconjugated (bs-6064R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6064R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3922R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4\u00b0C, DAB staining.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (bs-3787R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3787R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Rat brain and heart lysates probed with CD1d1 Polyclonal Antibody, unconjugated (bs-3690R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:3000 for 90 minutes at 37\u00b0C.\\n
Mouse spleen cells probed with \\tCD1d1 Polyclonal Antibody, Unconjugated (bs-3690R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-3690R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IRF7 Polyclonal Antibody, Unconjugated (bs-2994R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IRF7 Polyclonal Antibody, Unconjugated (bs-2994R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRF7 Antibody(bs-2994R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2994R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse heart lysates probed with SPHK1 Polyclonal Antibody, unconjugated (bs-2652R) at 1:30 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Kidney tissue lysates probed with SPHK1 Polyclonal Antibody, Unconjugated (bs-2652R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2652R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Raw264.7 cell lysates probed with Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Rat splenocytes stained with Anti- TLR6\/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated (bs-2716R-PE-Cy5.5) at 1:25.
Rat splenocytes stained with Anti- TLR6\/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated (bs-2716R-PE-Cy5.5) at 1:25
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TLR6 Polyclonal Antibody, Unconjugated (bs-2716R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with TLR6 Polyclonal Antibody, Unconjugated (bs-2716R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-2716R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Bdkrb2\/B2R Polyclonal Antibody, Unconjugated (bs-2422R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse brain cells probed with Rabbit Anti-Bdkrb2 Polyclonal Antibody (bs-2422R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).

Catalog #1: bs-2422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human osteosarcoma tissue labeled with Anti-Twist Polyclonal Antibody (bs-2441R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TWIST1\/2 Polyclonal Antibody, Unconjugated (bs-2441R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2441R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Human U937 cells probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human A549 cell lysates probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2095r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- 5-HTR3Polyclonal Antibody, Unconjugated(bs-2126R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Spinal cord lysates; Lane 4: Mouse Heart lysates ; Lane 5: Rat Heart lysates probed with 5-HTR3\/HTR3A Polyclonal Antibody, Unconjugated (bs-2126R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2126R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse bone lysates probed with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HEK 293 stained with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:500 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Catalog #1: bs-20297R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2047R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1:rat heart lysates Lane 2: rat brain lysates probed with Anti A2AR\/Adenosine A2a receptor Polyclonal Antibody, Unconjugated (bs-1456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-A2AR\/Adenosine A2a Polyclonal Antibody, Unconjugated (bs-3927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1456R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining

Catalog #1: bs-11770R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody, Unconjugated (bs-1186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Image kindly submitted by Brittany Baldwin-Murphy from Cornell University.\\n\\nChick embryo liver hepatocytes probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody at 1:100 or 1:500.\\n
Mouse liver cells probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody (bs-1186R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary (bs-0295G-PE) at 1:100 (green) for 40 minutes compared to control cells (blue).
HepG2 lysates probed with Scavenger receptor class B member 1 Polyclonal Antibody, unconjugated (bs-1186R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-1186R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated