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Western Blot Antibodies



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Catalog #1: bs-2875R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-2875R-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-2875R-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2875R-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-2875R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2875R-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-2875R-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-2875R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-2875R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10940R-APC-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-10940R-APC-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-10940R-RBITC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-10940R-BF555

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-10940R-BF680

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-10940R-APC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC

Catalog #1: bs-10940R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10940R-BF750

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-10940R-PE-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-10940R-BF488

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-10940R-PerCP

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-10940R-PerCP-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-10940R-PE-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-10940R-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-10940R-BF647

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-10940R-Biotin

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10940R-BF594

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-10940R-PE-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-10940R-HRP

Applications: WB

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-10940R-BF350

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-10940R-FITC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-10940R-BF405

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-10940R-Cy3

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-10940R-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-10940R-PE

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52204r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

The Hela ( treated with 200J\/m2 UV-C then recovery for 30 min) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-p-Hsp27 (S78) antibody (bsm-52167R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Hela (H) cells were treated with or without UV (40 mJ\/cm2) for 30 min and anisomycin (25 \u03bcg\/mL) for 30 min , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-HSP27 (Ser78) monoclonal antibody, unconjugated (bsm-52167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSP27 (Ser78) Monoclonal Antibody, Unconjugated (bsm-52167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSP27 (Ser78) Monoclonal Antibody, Unconjugated (bsm-52167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52167r

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60717r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HeLa, (+): HeLa+ Human \u03b1-TNF \r\n(20ng\/ml, 30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): C6, (+): C6+ Calyculin A (100nM, \r\n30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 \r\nU\/ml) at 37\u2103 for 1 hour\r\nSection type: Formalin-fixed & Paraffin-embedded section \r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60711R
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline \r\nphosphatase (50 U\/ml) at 37\u2103 for 1 hour\r\nSection type: Formalin-fixed & Paraffin-embedded section \r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60711R

Catalog #1: bsm-60711r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60679r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60678r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60662r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60659r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-60661r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60659r-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60659r-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-60659r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-60659r-bf750-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60659r-apc-100ul

Applications: WB

Reactivity: Human

Conjugate: APC