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Western Blot Antibodies



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Catalog #1: bs-2760R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin labeled with Anti-CK10 Polyclonal Antibody, Unconjugated(bs-2700R) followed by conjugation to the secondary antibody and DAB staining
Mouse skin lysate probed (bs-2700R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CK10) polyclonal Antibody, Unconjugated (bs-2700R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK10 Polyclonal Antibody(bs-2700R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2700R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse spleen cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44v4 Antibody\uff08bs-2783R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44v4 Polyclonal Antibody(bs-2783R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Tissue\/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (bs-2783R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (bs-2783R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2783R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CLEC1\/CLEC1A Polyclonal Antibody, Unconjugated (bs-2542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Cerebrum lysates probed with CLEC1\/CLEC1A Polyclonal Antibody, Unconjugated (bs-2542R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2542R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, GuineaPig

Conjugate: Unconjugated

Catalog #1: bs-2646R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-2754R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2752R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-CD44v7 Polyclonal Antibody, Unconjugated (bs-2779R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Hela cell lysates; Lane 2: Human PANC-1 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human A549 cell lysates probed with CD44v7 Polyclonal Antibody, Unconjugated (bs-2779R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2779R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2615R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P105 RB Polyclonal Antibody, Unconjugated (bs-2777R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Thymus tissue lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with Rb Polyclonal Antibody, Unconjugated (bs-2777R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2777R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2422R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-2527R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-2605R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Antigen: bs-2444P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm.\\n
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Pleiotrophin) Polyclonal Antibody, Unconjugated (bs-2444R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Pleiotrophin) Polyclonal Antibody, Unconjugated (bs-2444R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Pleiotrophin) Polyclonal Antibody, Unconjugated (bs-2444R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2444R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2658R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-2389R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-2386r-hrp-100ul

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2358R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2493R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2413R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bs-2413R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2413R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2519R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2176R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2352R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2101R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-2488R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2379R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2170R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2072R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-2343R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2343R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2067R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2242R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-1970R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1970R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-2162R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Dog

Conjugate: Biotin

Catalog #1: bs-2194R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bs-2371R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2190R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2284R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bs-2189R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2116R

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-2115R

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-2081R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-19199R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Liver lysates probed with Angiotensin III Polyclonal Antibody, Unconjugated (bs-20101R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Angiotensin 3 Polyclonal Antibody(bs-20101R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-20101R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-HSD11B2 Polyclonal Antibody, Unconjugated (bs-20186R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSD11B2 Polyclonal Antibody(bs-20186R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Stomach tissue lysates probed with HSD11B2 Polyclonal Antibody, Unconjugated (bs-20186R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20186R

Applications: WB, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated