Human Survivin (Surv) ELISA Kit
Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surv. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Surv. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Surv, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surv in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Target Information
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126, PubMed:25778398, PubMed:28218735). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
| GENE ID | 332 |
| SWISS PROT | O15392 |
| SYNONYMS |
BIRC5; API4; EPR-1; Baculoviral Inhibitor Of Apoptosis Repeat Containing 5; Apoptosis Inhibitor 4; Survivin Variant 3 Alpha |
Materials Supplied
| Kit Components | 96 Wells Quantity/Size |
|---|---|
| Pre-coated, ready-to-use 96-well strip plate | 1 plate |
| Plate sealer for 96 wells | 2 |
| Standard |
2 tubes |
| Diluent buffer | 1 bottle |
| Detection Reagent A | 1 bottle |
| Detection Reagent B | 1 bottle |
| TMB Substrate | 1 tube |
| Stop Solution | 1 tube |
| Wash Buffer (30 ℅ concentrate) | 1 tube |
| Product data sheet | 1 copy |
Storage
| Storage | The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C. |
Performance Characteristics
| REPEATABILITY |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Surv were tested 20 times on one plate, respectively. |
| SENSITIVITY | The minimum detectable dose was 12.5pg/mL. |
| ASSAY RANGE | 31.2-2000pg/mL |
| SPECIFICITY | This assay has high sensitivity and excellent specificity for the detection of Surv. No significant cross-reactivity or interference between Surv and analogs was observed. Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between Surv and all analogs, therefore, cross reactivity may still exist. |
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