Mouse Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit
Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tie1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Tie1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Tie1, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tie1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Target Information
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
| GENE ID | 21846 |
| SWISS PROT | Q06806 |
| SYNONYMS |
JTK14; Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1; Tyrosine-protein kinase receptor Tie-1 |
Materials Supplied
| Kit Components | 96 Wells Quantity/Size |
|---|---|
| Pre-coated, ready-to-use 96-well strip plate | 1 plate |
| Plate sealer for 96 wells | 2 |
| Standard |
2 tubes |
| Diluent buffer | 1 bottle |
| Detection Reagent A | 1 bottle |
| Detection Reagent B | 1 bottle |
| TMB Substrate | 1 tube |
| Stop Solution | 1 tube |
| Wash Buffer (30 ℅ concentrate) | 1 tube |
| Product data sheet | 1 copy |
Storage
| Storage | The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C. |
Performance Characteristics
| REPEATABILITY |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Tie1 were tested 20 times on one plate, respectively. |
| SENSITIVITY | The minimum detectable dose was 0.247ng/mL. |
| ASSAY RANGE | 0.625-40ng/mL |
| SPECIFICITY | This assay has high sensitivity and excellent specificity for the detection of Tie1. No significant cross-reactivity or interference between Tie1 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between Tie1 and all analogs, therefore, cross reactivity may still exist. |
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