Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit
Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRPM4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TRPM4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain TRPM4, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TRPM4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Target Information
Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed:17188667, PubMed:29211714). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway (By similarity). Essential for the migration but not the maturation of dendritic cells (PubMed:18758465). Plays a role in keratinocyte differentiation (By similarity).
| GENE ID | 68667 |
| SWISS PROT | Q7TN37 |
| SYNONYMS |
TRPM4B; Melastatin-4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4 |
Materials Supplied
| Kit Components | 96 Wells Quantity/Size |
|---|---|
| Pre-coated, ready-to-use 96-well strip plate | 1 plate |
| Plate sealer for 96 wells | 2 |
| Standard |
2 tubes |
| Diluent buffer | 1 bottle |
| Detection Reagent A | 1 bottle |
| Detection Reagent B | 1 bottle |
| TMB Substrate | 1 tube |
| Stop Solution | 1 tube |
| Wash Buffer (30 ℅ concentrate) | 1 tube |
| Product data sheet | 1 copy |
Storage
| Storage | The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C. |
Performance Characteristics
| REPEATABILITY |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level TRPM4 were tested 20 times on one plate, respectively. |
| SENSITIVITY | The minimum detectable dose was 0.048ng/mL. |
| ASSAY RANGE | 0.156-10ng/mL |
| SPECIFICITY | This assay has high sensitivity and excellent specificity for the detection of TRPM4. No significant cross-reactivity or interference between TRPM4 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between TRPM4 and all analogs, therefore, cross reactivity may still exist. |
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