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ChRM2 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-0441R-Cy5
Product Name ChRM2 Polyclonal Antibody, Cy5 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Chicken
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ChRM2
Immunogen Range 168-250/466
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1129
Swiss Prot P08172
Subcellular location Cell membrane
Synonyms HM2; Muscarinic acetylcholine receptor M2; CHRM2
Background The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200