TRIM32/BBS11 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
Overview
Catalog # bs-0964R-A350
Product Name TRIM32/BBS11 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat
Specifications
Conjugation ALEXA FLUOR® 350
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse TRIM32
Immunogen Range 301-400/655
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 22954
Subcellular location Cytoplasm, Nucleus
Synonyms 72 kda Tat interacting Protein; BBS11; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H autosomal recessive; Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; Tripartite Mot Containing Protein 32; Zinc Finger Protein HT2A; TRI32_MOUSE.
Background Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) protein family. TRIM32, like all TRIM proteins, contains a domain structure composed of a B-box, a RING-finger and a coiled-coil motif. Additionally, TRIM32 has six C-terminal NHL domains; it is expressed mainly in the skeletal muscle. The TRIM32 gene encodes an E3 ubiquitin ligase, a protein that attaches ubiquitin to a lysine residue on a target protein and acts in conjunction with ubiquitin-conjugating enzymes UbcH5a, UbcH5c and UbcH6. Mutations in the TRIM32 gene cause two forms of autosomal recessive muscular dystrophy designated limb girdle muscular dystrophy type 2H (LGMD2H) and sarcotubular myopathy (STM). TRIM32 mutations can also result in Bardet-Biedl syndrome (BBS), an autosomal recessive disorder characterized by pigmentary retinopathy, polydactyly, hypogenitalism, renal abnormalities, learning disabilities and obesity.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200