Overview |
bs-10180R-Biotin |
Adeno-Associated Virus Capsid Protein VP1 Antibody, Biotin Conjugated |
ELISA, IHC-P, IHC-F |
This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13 |
Virus, Others |
Specifications |
Biotin |
Rabbit |
KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1 |
21-120/724 |
Polyclonal |
IgG |
1ug/ul |
Purified by Protein A. |
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Store at -20°C for 12 months. |
Target |
capsid protein Adeno-associated 5 virus; capsid protein [Adeno-associated virus - 5]; capsid protein AAV5; Parvovirus coat protein VP1; capsid protein. |
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus |
Application Dilution |
ELISA |
1:500-1000 |
IHC-P |
1:200-400 |
IHC-F |
1:100-500 |