CPA3/MC-CPA Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-12338R-HRP
Product Name CPA3/MC-CPA Polyclonal Antibody, HRP Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Rat
Predicted Reactivity Human, Mouse
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CPA3/MC-CPA
Immunogen Range 101-200/417
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin.
Storage Condition Store at 4°C for 12 months.
Target
Gene ID 1359
Subcellular location Cytoplasm
Synonyms carboxypeptidase A; Carboxypeptidase A3; carboxypeptidase A3 mast cell; CBPA3_HUMAN; Cpa3; Mast cell carboxypeptidase A; mast cell carboxypeptidase A3; MC CPA; MC-CPA.
Background Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.
Application Dilution
WB 1:300-1000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500