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cdc25C (Ser216) Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-3096R-A555
Product Name cdc25C (Ser216) Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This phosphorylation site is found at Ser173 in Isoform 3 of this protein.
Reactivity Human, Mouse, Rat
Specifications
Conjugation ALEXA FLUOR® 555
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216
Modification Site Ser216
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 995
Swiss Prot P30307
Subcellular location Nucleus
Synonyms CDC25; PPP1R60; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C
Background Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200