Ephrin B (Tyr324 + Tyr329) Antibody, ALEXA FLUOR® 350 Conjugated

Applications

WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-3129R-A350
Product Name	Ephrin B (Tyr324 + Tyr329) Antibody, ALEXA FLUOR® 350 Conjugated
Applications	WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	These phosphorylation sites are homologous to Tyr323 + Tyr328 in Mouse.
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	ALEXA FLUOR® 350
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr324/329
Modification Site	Tyr324 + Tyr329
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	1947
Swiss Prot	P98172
Subcellular location	Cell membrane
Synonyms	CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2; Ephrin-B1; EFL-3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB1
Background	Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
Application Dilution
WB	1:300-5000
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Search our catalog of hundreds of thousands of products