GPR15 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated

$Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-GPR15 Antibody, conjugated (bs-3699R-AF488) 1:400, 90 minutes at 37\u00b0C; DAPI (5ug\/ml, blue,) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-GPR15 Antibody, conjugated (bs-3699R-AF488) 1:400, 90 minutes at 37\u00b0C; DAPI (5ug\/ml, blue,) was used to stain the cell nuclei.

Applications

WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Horse
Rabbit

Overview
Catalog #	bs-3699R-A488
Product Name	GPR15 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
Applications	WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Horse, Rabbit
Specifications
Conjugation	ALEXA FLUOR® 488
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GPR15
Immunogen Range	51-150/360
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	2838
Swiss Prot	P49685
Subcellular location	Cytoplasm, Cell membrane
Synonyms	BOB; G-protein coupled receptor 15; Brother of Bonzo; GPR15
Background	GPR15 is a probable chemokine receptor,its expression has been reported in CD4(+) T lymphocytes, alveolar macrophages, and the basal surface of the small intestinal epithelium. ESTs have been isolated from normal brain and kidney cancer libraries.This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012].
Application Dilution
WB	1:300-5000
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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