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Cdc25B (Ser186) Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
Overview
Catalog # bs-5244R-Biotin
Product Name Cdc25B (Ser186) Polyclonal Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser186
Modification Site Ser186
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 994
Swiss Prot P30305
Subcellular location Cytoplasm
Synonyms M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2
Background Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500