Mycobacterium tuberculosis Ag85A/B Antibody

Applications

  • IHC-P
  • IF(IHC-P)

Reactivity

  • Bacteria
Overview
Catalog # bs-6460R
Product Name Mycobacterium tuberculosis Ag85A/B Antibody
Applications IHC-P, IF(IHC-P)
Specificity This antibody will detect both Mycobacterium tuberculosis Ag85A and Mycobacterium tuberculosis Ag85B
Reactivity Bacteria
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Antigen 85 complex B
Immunogen Range 100-150/325
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID 885785, 886132
Swiss Prot P9WQP1, P9WQP3
Subcellular location Secreted
Synonyms M.tuberculosis;Antigen 85 complex B;30 kDa extracellular protein;Mycobacterium;Mycolyl transferase 85B; A85B_MYCTU;Antigen 85-B; 30 kDa extracellular protein; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B.
Background The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM
Application Dilution
IHC-P 1:200-400
IF(IHC-P) 1:50-200