Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEFb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DEFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain DEFb1, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DEFb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Has bactericidal activity. Active against E.coli ML35 but not against S.aureus 502A (PubMed:8454635). May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (By similarity).
B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1
|Kit Components||96 Wells Quantity/Size|
|Pre-coated, ready-to-use 96-well strip plate||1 plate|
|Plate sealer for 96 wells||2|
|Diluent buffer||1 bottle|
|Detection Reagent A||1 bottle|
|Detection Reagent B||1 bottle|
|TMB Substrate||1 tube|
|Stop Solution||1 tube|
|Wash Buffer (30 ℅ concentrate)||1 tube|
|Product data sheet||1 copy|
|Storage||The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C.|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level DEFb1 were tested 20 times on one plate, respectively.
|SENSITIVITY||The minimum detectable dose was 0.71ng/mL.|
|SPECIFICITY||This assay has high sensitivity and excellent specificity for the detection of DEFb1.
No significant cross-reactivity or interference between DEFb1 and analogs was observed.
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between DEFb1 and all analogs, therefore, cross reactivity may still exist.