Human MCP-1 ELISA Kit
Due to the possibility of mismatching between antigens from other origin and antibodies used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
Principle of the Assay
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MCP-1 is added to the wells and binds to the combination of capture antibody- MCP-1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of MCP-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MCP-1 standard dilutions and MCP-1 sample concentration determined.
For use with: Serum, Plasma, and Cell culture supernatants.
For Research Use Only. Not for use in diagnostic procedures.
Target Information
Monocyte chemoattractant protein-1 (MCP-1) called also monocyte chemotactic protein-1. MCP-1 is an 8.6kDa protein and belongs to the family of chemotactic cytokines known as Chemokines. MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells, and human pulmonary type-2-like epithelial cells in culture. MCP-1 exhibits chemotactic activity for monocytes/macrophages, basophils, T lymphocytes, particularly memory T cells and natural killer cells and neural stem cells. Elevated levels of MCP-1 are detected during inflammation and immune responses. MCP-1 activity is also important in wound healing as shown by delayed wound repair in MCP-1deficient mice. MCP-1 is implicated in the pathogenesis of disease states that involve monocyte/macrophage infiltrates, such as psoriasis, rheumatoid arthritis and recruitment of monocytes to the arterial wall in atherosclerosis. In addition to its chemotactic function, MCP-1 also induces the expression of IL-10 from macrophages, which favours a TH2 immune response. MCP-1 is a glycopeptide containing one potential N-linked glycosylation site. The extent of MCP-1 glycosylation influences its chemotactic potency and half-life in vivo.
| GENE ID | 6347 |
| SWISS PROT | P13500 |
| SYNONYMS | HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30 |
Materials Supplied
| Kit Components | 96 Wells Quantity/Size |
|---|---|
| Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human MCP-1 (8﹡12) | 1 plate |
| Human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution | 2 vials |
| Concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody | 2 vials |
| Streptavidin-HRP solution | 2 vials |
| Standard /sample Diluent | 1 bottle |
| Biotin-Conjugate antibody Diluent | 1 bottle |
| Streptavidin-HRP Diluent | 1 bottle |
| Wash Buffer Concentrate 20x (PBS with 1% Tween-20) | 1 bottle |
| Substrate Solution | 1 vial |
| Stop Solution | 1 vial |
| Adhesive Films | 4 pieces |
| Product data sheet | 1 copy |
Storage
| Storage | Store at 2 - 8°C |
Performance Characteristics
| REPEATABILITY | The coefficient of variation of both intra-assay and inter-assay were less than 10%. |
| SENSITIVITY | The minimum detectable dose was 4pg/mL. |
| ASSAY RANGE | 15.6–1,000pg/mL |
| SPECIFICITY | This assay recognizes both natural and recombinant human MCP-1. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed. |
Frequently asked questions
Which species does Human MCP-1 ELISA Kit (BSKH1040) detect?
BSKH1040 is designed for Human samples.