SARS-CoV-2 Total Antibody ELISA Kit

Principle of the Assay

The kit uses enzyme-linked immunoassay. According to the principle of antibody blocking; the following design was made: The recombinant protein of SARS-CoV-2-N was used as a coating antigen and was coated with a 96-well reaction plate. When the positive sample is added and within a certain reaction time; the (solid-phase antigen-antibody) complex is formed. Wash the plate; get rid of the substance that didn't participate in the reaction. HRP anti-SARS-CoV-2-N antibody was added as a tracer. Antigenic reaction with no binding antibody on the reaction plate; Formation of (Solid-phase antigen - HRP antibody) complexes. Absorbance was measured 450nm using a microplate reader. The concentration of specific total antibody in the sample was inversely proportional to the absorbance (OD value).

Target Information

 Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by infection with the SARS-CoV-2 virus. Common signs of infection include respiratory symptoms, fever, cough, shortness of breath and breathing difficulties. In severe cases, the infection can cause pneumonia, severe acute respiratory syndrome (SARS), kidney failure and death. Coronaviruses (CoV) are a large family of viruses that cause illness ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). SARS-CoV-2 is a new strain of coronavirus that was first identified in December 2019 when the first outbreak occurred in Wuhan, China.

Materials Supplied

Kit Components 96 Wells Quantity/Size
Microplate coated with SARS-CoV-2-N recombinant protein antigen. (8×12)
1 plate
Negative reference product; Specification: 0.8ml / bottle
1 bottle
Positive reference product;  Specification: 0.8ml / bottle
1 bottle
Enzyme Conjugate: anti-SARS-CoV-2-N monoclonal antibody with HRP marker: Specification: 10.5ml / bottle.
1 bottle
Color rendering solution A, mainly composed of H2O2; Specification: 5.0ml / bottle. 1 bottle
Color rendering solution B, mainly composed of which is TMB. Specification: 5.0ml / bottle.
1 bottle
Termination solution, mainly containing dilute H2SO4. Specification: 5.0ml / bottle. 1 bottle
Concentrated washing solution, 10 times concentrated washing solution, diluted before use. Specification: 50.0ml / bottle.
1 bottle
Operating instruction
1 copy

Storage

Storage Store at 2 - 8°C

Performance Characteristics

REPEATABILITY Tested the positive reference products of the enterprise. Repeated detection 10 times, all the results were positive.
SPECIFICITY Review the datasheet for extended details.