SARS-CoV-2 Total Antibody Detection ELISA Kit (Targeting RBD of Spike Proteins)
Due to the possibility of mismatching between antigens from other origin and antibodies used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
Principle of the Assay
This assay employs a quantitative sandwich enzyme immunoassay technique and is uniquely suitable for rapid high-throughput detecting the levels of the SARS-CoV-2 total antibody in serum. The recombinant SARS-CoV-2 RBD fragment has been pre-coated onto a microplate. Controls or samples are pipetted into the wells, and then a horseradish peroxidase-conjugated RBD fragment (RBD-HRP) is added to the wells, producing an antigen-antibody-antigen "sandwich complex". Following incubation and wash steps, a substrate is added. A colored product is formed in proportion to the amount of SARS-CoV-2 total antibody present in the sample. The reaction is terminated by the addition of acid and absorbance is measured at 450nm. A positive control is prepared from SARS-CoV-2 RBD antibodies to assure the validity of the results.
Target Information
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. SARS-CoV-2 has several structural proteins including spike (S), envelope (E), membrane (M), and nucleocapsid (N). The spike protein (S) contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor, angiotensin-converting enzyme-2 (ACE2). It is found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2 receptor leading to endocytosis into the host cells of the deep lung and viral replication. This kit is configured and optimized to support research for SARS-CoV-2, it can be used to detect the RBD antibodies in the serum of patients with SARS-CoV-2 infection as early as possible, and serving as a tool to evaluate the immune level of other animals to the SARS-COV-2 RBD.
Materials Supplied
Kit Components | 96 Wells Quantity/Size |
---|---|
Aluminum pouches with a Microwell Plate coated with SARS-CoV-2 RBD fragment (8×12) |
1 plate |
Positive Control | 2 vials |
Negative Control | 2 vials |
Recombinant SARS-CoV-2 RBD fragment conjugated to horseradish peroxidase (HRP) |
2 vials |
Diluent Buffer |
1 bottle |
Wash Buffer Concentrate 20x (PBS with 1% Tween-20) | 1 bottle |
Substrate Solution | 1 bottle |
Stop Solution | 1 bottle |
Adhesive Films | 4 pieces |
Product insert | 1 copy |
Storage
Storage | Store at 2 - 8°C |
Performance Characteristics
Repeatability | The coefficient of variation of both intra-assay and inter-assay was less than 10%. |
Specificity | This assay is specific to SARS-CoV-2 neutralizing antibodies. |
Method Verification | To evaluate the sensitivity of the SARS-CoV-2 Total Antibody Detection ELISA Kit, positive serum and negative serum were tested by diluted in serial two-fold steps in Diluent Buffer. The figure(below) shows the results with the SARS-CoV-2 Total Antibody Detection ELISA Kit for tested samples: |