Bak (7A12) Monoclonal Antibody

$Lane 1: Hela cells; Lane 2: Human skeletal muscle; Lane 3: Ags cells; Probed with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells; Lane 2: Human skeletal muscle; Lane 3: Ags cells; Probed with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Flow cytometric analysis of NIH\/3T3 cells with Bak (7A12) Monoclonal Antibody (bsm-52415R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of NIH\/3T3 cells with Bak (7A12) Monoclonal Antibody (bsm-52415R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52415R
Product Name	Bak (7A12) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Bak between 1 to 100 amino acids
Clonality	Monoclonal
Clone #	7A12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	578
Swiss Prot	Q16611
Synonyms	Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1
Background	Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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