TOMM20 (32A1) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).

$Lane 1: Hela cells; Lane 2: MCF-7 cells; Lane 3: F9 cells; Lane 4: PC12 cells; Probed with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells; Lane 2: MCF-7 cells; Lane 3: F9 cells; Lane 4: PC12 cells; Probed with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52449R
Product Name	TOMM20 (32A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C-terminus Human TOMM20
Clonality	Monoclonal
Clone #	32A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	9804
Swiss Prot	Q15388
Synonyms	Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20
Background	Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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