DKK1 (1B10) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$MCF-7 cell lysate probed with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

MCF-7 cell lysate probed with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Rat

Overview
Catalog #	bsm-52532R
Product Name	DKK1 (1B10) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant Human DKK1 between 250-350 amino acids
Clonality	Monoclonal
Clone #	1B10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	22943
Swiss Prot	O94907
Synonyms	Dickkopf-related protein 1, SK, Dickkopf-1, Dkk-1, hDkk-1, DKK1
Background	Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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