Myosin Light Chain 2 (10B7) Monoclonal Antibody

$Lane 1: Mouse liver; Lane 2: Mouse brain; Probed with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse liver; Lane 2: Mouse brain; Probed with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52575R
Product Name	Myosin Light Chain 2 (10B7) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Myosin Light Chain 2 between 150-250 amino acids
Clonality	Monoclonal
Clone #	10B7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	17906
Swiss Prot	P51667
Synonyms	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, Cardiac myosin light chain 2, Myosin light chain 2, slow skeletal/ventricular muscle isoform, Ventricular myosin light chain 2, MLC-2, MLC-2v, MLC-2s/v, MYL2
Background	Contractile protein that plays a role in heart development and function (PubMed:10409661). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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