RAD18 (9G8) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: NIH\/3T3 Cells; Lane 2: 293T Cells; Lane 3: MCF-7 Cells; Probed with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: NIH\/3T3 Cells; Lane 2: 293T Cells; Lane 3: MCF-7 Cells; Probed with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:300 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:300 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat testis tissue incubated with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat testis tissue incubated with RAD18 (9G8) Monoclonal Antibody (bsm-52590R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52590R
Product Name	RAD18 (9G8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human RAD18 between 300-500 amino acids
Clonality	Monoclonal
Clone #	9G8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	56852
Swiss Prot	Q9NS91
Synonyms	E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, RING finger protein 73, hHR18, hRAD18, RAD18
Background	E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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