NSD3 (5H4) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with NSD3 (5H4) Monoclonal Antibody (bsm-52597R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: MCF-7 Cells; Lane 2: 293T Cells; Probed with NSD3 (5H4) Monoclonal Antibody (bsm-52597R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Lane 2: 293T Cells; Probed with NSD3 (5H4) Monoclonal Antibody (bsm-52597R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52597R
Product Name	NSD3 (5H4) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human NSD3 aa 1-100
Clonality	Monoclonal
Clone #	5H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	54904
Swiss Prot	Q9BZ95
Synonyms	Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1, WHSC1-like protein 1_ WHSC1L1
Background	Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Application Dilution
WB	1:300-5000
FCM	1:20-100

Applications

Reactivity

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