Caspase-10 (3C5) Monoclonal Antibody

Flow cytometric analysis of K562 cells with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$K562 cell lysate probed with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

K562 cell lysate probed with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-52740R
Product Name	Caspase-10 (3C5) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Caspase-10 aa 1-250
Clonality	Monoclonal
Clone #	3C5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	843
Swiss Prot	Q92851
Synonyms	Caspase-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, ICE-like apoptotic protease 4, CASP-10, FLICE2, CASP10
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-\|-AMC and Asp-Glu-Val-Asp-\|-AMC. Isoform C is proteolytically inactive.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

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