SUMO4 (3C3) Monoclonal Antibody

Flow cytometric analysis of 293 cells with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: 293T Cells; Lane 2: Jurkat Cells; Probed with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293T Cells; Lane 2: Jurkat Cells; Probed with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUMO4 (3C3) Monoclonal Antibody (bsm-52767R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52767R
Product Name	SUMO4 (3C3) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human SUMO4 aa 1-100
Clonality	Monoclonal
Clone #	3C3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	387082
Swiss Prot	Q6EEV6
Synonyms	Small ubiquitin-related modifier 4, Small ubiquitin-like protein 4, SUMO-4, SUMO4
Background	Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200

Applications

Reactivity

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