PDIA6 (4C3) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: HepG2 lysates; Lane 2: K562 lysates; Probed with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 lysates; Lane 2: K562 lysates; Probed with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:300 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:300 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:300 in NIH\/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:300 in NIH\/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52808R
Product Name	PDIA6 (4C3) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human PDIA6 between 300 to 500 amino acids
Clonality	Monoclonal
Clone #	4C3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10130
Swiss Prot	Q15084
Synonyms	Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5, PDIA6
Background	May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed:24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed:24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200

Applications

Reactivity

Search our catalog of hundreds of thousands of products