VCP (8C1) Monoclonal Antibody

Flow cytometric analysis of HL-60 cells with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)

$Lane 1: SH-SY5Y cells lysates; Lane 2: HepG2 cells lysates; Line 3: Hela cells lysates; Probed with VCP (8C1)Monoclonal Antibody (bsm-52931R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n$

Lane 1: SH-SY5Y cells lysates; Lane 2: HepG2 cells lysates; Line 3: Hela cells lysates; Probed with VCP (8C1)Monoclonal Antibody (bsm-52931R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n

$Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with VCP (8C1) Monoclonal Antibody (bsm-52931R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52931R
Product Name	VCP (8C1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human VCP between 1-100 amino acids
Clonality	Monoclonal
Clone #	8C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7415
Swiss Prot	P55072
Synonyms	Transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, TER ATPase, VCP
Background	Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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