MIF (8C2) Monoclonal Antibody

$Lane 1: Mouse brain cells lysates; Lane 2: Jurkat cells lysates; Line 3: Hela cells lysates;Probed with MIF (8C2) Monoclonal Antibody (bsm-52964R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n$

Lane 1: Mouse brain cells lysates; Lane 2: Jurkat cells lysates; Line 3: Hela cells lysates;Probed with MIF (8C2) Monoclonal Antibody (bsm-52964R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52964R
Product Name	MIF (8C2) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal human MIF
Clonality	Monoclonal
Clone #	8C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4282
Swiss Prot	P14174
Synonyms	Macrophage migRation inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, MIF, GIF
Background	Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Application Dilution
WB	1:300-5000

Applications

Reactivity

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