DNA Polymerase beta (1C7) Monoclonal Antibody

$Lane 1: PC-12 Cells; Lane 2: NIH-3T3 Cells; Lane 3: A431 Cells; Probed with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-12 Cells; Lane 2: NIH-3T3 Cells; Lane 3: A431 Cells; Probed with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Zebrafish Tissue; Probed with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:200 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Zebrafish Tissue; Probed with DNA Polymerase beta (1C7) Monoclonal Antibody (bsm-54069R) at 1:200 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-54069R
Product Name	DNA Polymerase beta (1C7) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human DNA Polymerase beta aa 300 to the C-terminus
Clonality	Monoclonal
Clone #	1C7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5423
Swiss Prot	P06746
Synonyms	DNA polymerase beta, POLB
Background	Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

Search our catalog of hundreds of thousands of products