WDR5 (8G9) Monoclonal Antibody

$Lane 1: Hela Cells; Lane 2: A549 Cells; Lane 3: NIH-3T3 Cells; Lane 4: PC-12 Cells; Probed with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: A549 Cells; Lane 3: NIH-3T3 Cells; Lane 4: PC-12 Cells; Probed with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Hela cells with WDR5 (8G9) Monoclonal Antibody (bsm-54114R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54114R
Product Name	WDR5 (8G9) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human WDR5 aa 300 to the C-terminus
Clonality	Monoclonal
Clone #	8G9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	11091
Swiss Prot	P61964
Subcellular location	Nucleus
Synonyms	WD repeat-containing protein 5; BMP2-induced 3-kb gene protein; WDR5; BIG3; CFAP89; SWD3
Background	Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

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