Cyclophilin F (2A1) Monoclonal Antibody

$Lane 1: Human Heart; Lane 2: Human Brain; Probed with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Heart; Lane 2: Human Brain; Probed with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Cyclophilin F (2A1) Monoclonal Antibody (bsm-54124R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54124R
Product Name	Cyclophilin F (2A1) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Cyclophilin F aa 1-100
Clonality	Monoclonal
Clone #	2A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10105
Swiss Prot	P30405
Subcellular location	Mitochondrion
Synonyms	Peptidyl-prolyl cis-trans isomerase F mitochondrial; Cyclophilin D; Cyclophilin F; Mitochondrial cyclophilin, Rotamase F; PPIase F; CyP-D; CypD; CyP-M; PPIF; CYP3.
Background	PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis. Note: This gene encodes a 178 aa mature peptide that is found in the mitochondrion. It has been labeled 'cyclophilin D'. This same name has also been applied to a different cytoplasmic protein of 370 aa, which is represented by Entrez GeneID 5481, PPID.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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