HPRT1 (6C9) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Hela Cells; Lane 2: A431 Cells; Lane 3: MCF-7 Cells; Lane 4: 293 Cells; Lane 5: Rat Kidney; Lane 6: Rat Brain; Lane 7: Mouse Testis; Lane 8: Mouse Colon; Probed with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: A431 Cells; Lane 3: MCF-7 Cells; Lane 4: 293 Cells; Lane 5: Rat Kidney; Lane 6: Rat Brain; Lane 7: Mouse Testis; Lane 8: Mouse Colon; Probed with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Zebrafish Tissue; Probed with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Zebrafish Tissue; Probed with HPRT1 (6C9) Monoclonal Antibody (bsm-54160R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-54160R
Product Name	HPRT1 (6C9) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human HPRT aa 1-200
Clonality	Monoclonal
Clone #	6C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3251
Swiss Prot	P00492
Synonyms	Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase; HPRT1; hypoxanthine phosphoribosyltransferase 1; HPRT.
Background	Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this protein result in Lesch-Nyhan syndrome or gout.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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