NUP153 (6C1) Monoclonal Antibody

$Lane 1: K562 Cells; Lane 2: HepG2 Cells; Probed with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2: HepG2 Cells; Probed with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:200 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:200 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:200 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) at 1:200 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Flow cytometric analysis of K562 cells with NUP153 (6C1) Monoclonal Antibody (bsm-54175R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54175R
Product Name	NUP153 (6C1) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human Nup153 aa 50-100
Clonality	Monoclonal
Clone #	6C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	9972
Swiss Prot	P49790
Synonyms	Nuclear pore complex protein Nup153; 153 kDa nucleoporin; Nucleoporin Nup153; NUP153.
Background	Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200

Applications

Reactivity

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