Mad2L2 (1C4) Monoclonal Antibody

$Lane 1: SiHa Cells; Probed with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Probed with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Hela cells with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54188R
Product Name	Mad2L2 (1C4) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant full length protein of human Mad2L2
Clonality	Monoclonal
Clone #	1C4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10459
Swiss Prot	Q9UI95
Synonyms	Mitotic spindle assembly checkpoint protein MAD2B; Mitotic arrest deficient 2-like protein 2; REV7 homolog; MAD2-like protein 2; hREV7; MAD2L2; MAD2B; REV7.
Background	Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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