SF3B1 (6C6) Monoclonal Antibody

$Lane 1: Mouse Thymus; Lane 2: Hela Cells; Probed with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Thymus; Lane 2: Hela Cells; Probed with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54250R
Product Name	SF3B1 (6C6) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human SF3B1 aa 1-150
Clonality	Monoclonal
Clone #	6C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3451
Swiss Prot	O75533
Subcellular location	Nucleus
Synonyms	Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; Spliceosome-associated protein 155; SF3b155; SAP 155; SF3B1; SAP155.
Background	Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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