Apolipoprotein A2 (2H4) Monoclonal Antibody

$IF(ICC) staining with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:200 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:200 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:200 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:200 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:200 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:200 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Large Intestine tissue incubated with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Large Intestine tissue incubated with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Liver tissue incubated with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Liver tissue incubated with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of HepG2 cells with Apolipoprotein A2 (2H4) Monoclonal Antibody (bsm-54292R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Rat

Overview
Catalog #	bsm-54292R
Product Name	Apolipoprotein A2 (2H4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length recombinant protein of human Apolipoprotein A II
Clonality	Monoclonal
Clone #	2H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	336
Swiss Prot	P02652
Subcellular location	Secreted
Synonyms	Apolipoprotein A-II; Apolipoprotein A2; Proapolipoprotein A-II; Truncated apolipoprotein A-II; Apolipoprotein A-II(1-76); Apo-AII; ApoA-II; ProapoA-II; APOA2
Background	Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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