SMARCB1 (7G8) Monoclonal Antibody

$Lane 1: Daudi Cells; Lane 2: Mouse Lymph Node; Probed with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cells; Lane 2: Mouse Lymph Node; Probed with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of SH-SY5Y cells with SMARCB1 (7G8) Monoclonal Antibody (bsm-54327R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54327R
Product Name	SMARCB1 (7G8) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide of C-terminal Human SNF5
Clonality	Monoclonal
Clone #	7G8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6598
Swiss Prot	Q12824
Subcellular location	Nucleus
Synonyms	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; BRG1-associated factor 47; Integrase interactor 1 protein; SNF5 homolog; BAF47; hSNF5; SMARCB1; BAF47; INI1; SNF5L1
Background	Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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