Gephyrin (7A1) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: SK-BR-3 Cells; Lane 2: Rat Kidney; Probed with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SK-BR-3 Cells; Lane 2: Rat Kidney; Probed with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54343R
Product Name	Gephyrin (7A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Gephyrin aa 1-100.
Clonality	Monoclonal
Clone #	7A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10243
Swiss Prot	Q9NQX3
Subcellular location	Cell membrane, Cytoskeleton
Synonyms	Gephyrin; Molybdopterin adenylyltransferase; Domain G; Molybdopterin molybdenumtransferase; Domain E; MPT adenylyltransferase; MPT Mo-transferase; GPHN; GPH; KIAA1385
Background	The sub-membraneous region at the postsynaptic membrane contains a number of proteins critical for receptor targeting. Gephyrin is a microtubule-associated protein highly expressed in brain and localized to neuronal postsynaptic membranes. Gephyrin is essential for the postsynaptic localization of the inhibitory glycine receptor and is thought to anchor the receptor to subsynaptic microtubules. The protein is expressed in most mammalian tissues with predominant expression in brain. At least five additional splice variants of Gephyrin ranging in molecular weight have been identified in rat and human brain tissue.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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