ELMO1 (12A1) Monoclonal Antibody

$Lane 1: Mouse Spleen; Lane 2: Jurkat Cells; Probed with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Spleen; Lane 2: Jurkat Cells; Probed with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Spleen tissue incubated with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Spleen tissue incubated with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of SH-SY5Y cells with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54345R
Product Name	ELMO1 (12A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human ELMO1 aa 500 to 700.
Clonality	Monoclonal
Clone #	12A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	9844
Swiss Prot	Q92556
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Engulfment and cell motility protein 1; Protein ced-12 homolog; ELMO1; KIAA0281; CED12; CED-12
Background	Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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