SAE1 (1F1) Monoclonal Antibody

$Lane 1: SiHa Cells; Lane 2: K562 Cells; Lane 3: 293 Cells Probed with SAE1 (1F1) Monoclonal Antibody (bsm-54358R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Lane 2: K562 Cells; Lane 3: 293 Cells Probed with SAE1 (1F1) Monoclonal Antibody (bsm-54358R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of A549 cells with SAE1 (1F1) Monoclonal Antibody (bsm-54358R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54358R
Product Name	SAE1 (1F1) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human SAE1 aa 150-300.
Clonality	Monoclonal
Clone #	1F1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10055
Swiss Prot	Q9UBE0
Subcellular location	Nucleus
Synonyms	SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; SUMO-activating enzyme subunit 1; N-terminally processed; SAE1; AOS1; SUA1; UBLE1A
Background	The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200

Applications

Reactivity

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