NDUFB8 (25B3) Monoclonal Antibody

$Lane 1: 293 Cells; Lane 2: A549 Cells; Lane 3: Mouse Heart; Lane 4: Rat Spleen; Probed with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cells; Lane 2: A549 Cells; Lane 3: Mouse Heart; Lane 4: Rat Spleen; Probed with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of 293T cells with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54361R
Product Name	NDUFB8 (25B3) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human NDUFB8 aa 30-130.
Clonality	Monoclonal
Clone #	25B3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	4714
Swiss Prot	O95169
Subcellular location	Mitochondrion
Synonyms	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial; Complex I-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit; CI-ASHI; NDUFB8; NADH ubiquinone oxidoreductase subunit B8
Background	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

Search our catalog of hundreds of thousands of products